Research from the laboratory of Matthew Lev at Washington University in St. Louis offers entirely new ways to see the little one.
They have developed new hardware and algorithms that allow them to visualize the building blocks of the biological world beyond three dimensions in ways that until now have not been feasible. After all, cells are 3D objects and full of “things” – molecules – that move, twist, turn and tumble to drive life itself.
Like traditional microscopes, the work of two Lew Lab PhD students, Tingting Wu and Oumeng Zhang, uses light to peer into the microscopic world – but their innovations are anything but traditional. Currently, when people use light in imagery, they are probably interested in the brightness of that light or its color. But light has other properties, including polarization.
“Oumeng’s work distorts the polarization of light,” said Lew, assistant professor in the Department of Electrical and Systems Engineering at Preston M. Green. “That way you can see both how things translate (move in straight lines) and rotate at the same time” – something traditional imagery doesn’t.
“The development of new technologies and the ability to see things we couldn’t see before is exciting,” Zhang said. This unique ability to track both rotation and position gives it unique insight into how biological materials – human cells and pathogens, for example – interact.
Wu’s research also provides a new way to image cell membranes and, in a way, see inside them. Using fluorescent tracer molecules, she maps how tracers interact with fat and cholesterol molecules in the membrane, determining how lipids are arranged and organized.
“Any cell membrane, any nucleus, anything inside the cell is a 3D structure,” she said. “It helps us probe the full picture of a biological system. This allows us, for any biological sample, to see beyond three dimensions – we see 3D structure plus three dimensions of molecular orientation, giving us 6D images.
Researchers have developed computer imaging technology, which combines software and hardware, to successfully see what was previously invisible.
“That’s part of the innovation,” Lew said. “Traditionally, bioimaging labs were tied to whatever commercial manufacturers made. But if we think of things differently, we can do so much more.
This research was supported by the National Science Foundation, No. ECCS-1653777 and the National Institute of General Medical Sciences, No. R35GM124858.
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